The brief

Analyst view

• PacBio's HiFi long-read sequencing captures structural variants and repeats that short-read sequencers miss.
• Revio handles high-throughput research; Vega is a benchtop system for smaller labs with lower DNA input.
• Vega SPRQ-Nx chemistry (Aug 2026): 90 Gb per run, 40% lower cost, 500 ng DNA input vs. 2 µg prior.

Technical approach

As reported

Long reads

SMRT sequencing captures structural variation

Single-molecule real-time (SMRT) reads 10,000+ base fragments, revealing repetitive DNA and copy number variants that short-read sequencers cannot.

Accuracy

HiFi consensus exceeds 99.9%

Circular DNA templates allow multiple passes, with consensus calling achieving >99.9% accuracy without sacrificing read length or completeness.

Flexibility

Two platforms for different scales

Revio for research and diagnostics; Vega for smaller labs needing minimal DNA input and compact footprint.